Description
General description
This panel targets phosphorylated proteins in the TGFβ signaling pathway, which regulates cell growth, differentiation, and immune responses. SMAD2 (pS465/pS467) and SMAD3 (pS423/pS425) are direct mediators of TGFβ receptor activation. TAK1 (pS412), p38 (pT180/pY182), ERK1/2 (pT185/pY187), and JNK (pT183/pY185) represent downstream MAPKs involved in inflammation, fibrosis, and cancer progression.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in cell lysate samples.
Analytes Included: SMAD2 (Phospho-Ser465/467), SMAD3 (Phospho-Ser423/425), TAK1 (Phospho-Ser412), p38 (Phospho-Thr180/Tyr182), ERK1/2 (Phospho-Thr185/Tyr187), JNK (Phospho-Thr183/Tyr185)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect 6 phosphorylated proteins in the TGFβ signaling pathway
Includes SMAD2, SMAD3, TAK1, p38, ERK1/2, and JNK
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from fibrosis, inflammation, and cancer models
Ideal for fibrosis, immunology, and cell signaling research
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corporation
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
