Description
General description
This panel targets phosphorylated proteins involved in the regulation of protein synthesis. It includes p70S6K (pT412), 4E-BP1 (pT37/46), eIF2α (pS51), eIF4E (pS209), mTOR (pS2448), and RPS6 (pS235/236). These proteins are downstream effectors of the mTOR pathway and play critical roles in translation initiation, cell growth, and metabolism. The panel supports studies in cancer, metabolic disorders, and cellular stress responses.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in human, mouse, or rat cell lysate samples.
Analytes Included: p70S6K (Phospho-Thr412), 4E-BP1 (Phospho-Thr37/46), eIF2α (Phospho-Ser51), eIF4E (Phospho-Ser209), mTOR (Phospho-Ser2448), RPS6 (Phospho-Ser235/236)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect 6 phosphorylated proteins involved in protein synthesis regulation
Includes p70S6K, 4E-BP1, eIF2α, eIF4E, mTOR, and RPS6
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from cancer, metabolic, and stress response studies
Ideal for translational control, oncology, and mTOR pathway research
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
