Description
General description
This panel includes phosphorylated proteins central to T-cell receptor signaling and immune activation. CD3ζ (pY142) initiates TCR signaling upon antigen recognition. LAT (pY191) and SLP-76 (pY128) serve as scaffolding proteins for downstream signaling. Lck (pY394) and ZAP70 (pY319) are tyrosine kinases that propagate TCR signals. PLCγ1 (pY783) mediates calcium signaling, while ERK (pT185/pY187) links TCR activation to gene expression and cell proliferation.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 7 analytes in cell lysate samples.
Analytes Included: CD3ζ (pY142), LAT (pY191), Lck (pY394), PLCγ1 (pY783), ZAP70 (pY319), SLP-76 (pY128), ERK (pT185/pY187)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect 7 phosphorylated proteins involved in T-cell receptor signaling
Includes CD3ζ, LAT, Lck, ZAP70, PLCγ1, SLP-76, and ERK for comprehensive immune profiling
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from T-cell activation and immunotherapy studies
Ideal for adaptive immunity, immune modulation, and cell signaling research
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
