Description
General description
This panel targets phosphorylated proteins in the Ras-Raf signaling cascade, a key pathway in cell proliferation and oncogenesis. It includes c-Raf (pS338), MEK1 (pS217/pS221), ERK1/2 (pT185/pY187), and downstream effectors such as p90RSK (pT359/pS363). Dysregulation of this pathway is commonly associated with various cancers, making this panel ideal for oncology and signal transduction research.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in cell lysate samples.
Analytes Included: c-Raf (Phospho-Ser338), MEK1 (Phospho-Ser217/221), ERK1/2 (Phospho-Thr185/Tyr187), p90RSK (Phospho-Thr359/Ser363)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect 6 phosphorylated proteins in the Ras-Raf-MEK-ERK signaling cascade
Includes c-Raf, MEK1, ERK1/2, and p90RSK
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from cancer, proliferation, and signal transduction studies
Ideal for oncology, targeted therapy research, and MAPK pathway analysis
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures"
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use indiagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which containsfluorescently labeled microsphere beads authorized by Luminex Corporation) orusing this Assay Product in any manner, you are consenting and agreeing to bebound by the End User Terms and Conditions and the End User License Agreementavailable at http://support.diasorin.com/end-user-terms-and-conditions/. If youdo not agree to all of the terms and conditions, you must promptly return thisAssay Product for a full refund prior to using it in any manner.
