Merck, 14-1058, PvuRts1I (5-hmC Restriction Endonuclease)

General description
35 kDa
PvuRts1I is an enzyme isolated from the gram negative bacterium Proteus vulgaris that can specifically digest DNA containing 5-hmC (Szwagierczak et al., Nucleic Acids Research, 2011) while leaving cytosine and 5-mC intact. This endonuclease can be used to analyze 5-hmC patterns in mammalian genomes by cleave cutting both glucosylated and non-glucosylated 5-hmC containing DNA sequences.

Research studies of DNA methylation and gene expression support the presence of 5-hydroxymethylcytosine (5-hmC) as a sixth base in mammalian genomes. 5-hmC is generated by Ten Eleven Translocation (TET) family of enzymes through oxidation of 5-methylcytosine (5-mC). Chemically, 5-hmC differs from 5-mC by the presence of a hydroxyl on the methyl group at position 5 of cytosine.
Physical form
Materials Provided:
PvuRts1I 5-hmC Endonuclease: 100U (1U/µL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol.

PvuRts1I 5-hmC 10X Reaction Buffer: 500µL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT.
Preparation Note
Stable at -20°C for 1 year from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.