Merck, 14-917, CLK4 Protein, active, 10 µg

General description
CLK4 protein belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined.
Product Source: N-terminal 6His-tagged, recombinant, human CLK4 amino acids 128-end expressed by baculovirus in Sf21 insect cells.
Application
Research Category
Signaling
Biochem/physiol Actions
Protein Target: CLK4
Target Sub-Family: CMGC
Packaging
Also available in 250μg size as catalog # 14-917M and in bulk as catalog # 14-917-K.
Physical form
50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Purified using immobilized metal affinity chromatography.
Preparation Note
6 months at -70°C
Analysis Note
Routinely evaluated by phosphorylation of peptide YRRAAVPPSPSLSRHSSPHQS(p)EDEEE.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.