Merck, 14-770, mTOR (1362-end) Protein, active, 10 µg

Name: mTOR (1362-end) Protein, active, 10 µg
Brand: Merck
SKU: 14-770

Size:10 μg, FRAP1, Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.

Available Catalog Numbers

14-1048 14-1057 14-1058 14-1067 14-1109 14-1121 14-155 14-173 14-197 14-197M 14-198 14-249 14-251 14-251M 14-276 14-276M 14-278 14-279 14-306 14-306M 14-309 14-314 14-314M

Description

General description
Research area: Cell Signaling

Protein Target: mTOR (FRAP1)

Target Sub-Family: Lipid/Atypical

N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end

The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Application
The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.
Biochem/physiol Actions
Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy. Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.
Packaging
Also available in 100μg size, please inquire for pricing and availability.
Physical form
16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol
Preparation Note
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Other Notes
Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Specifications

General

UNSPSC Code	12352202
NACRES	NA.41
eCl@ss	32160405
biological source	human
recombinant	expressed in baculovirus infected Sf21 cells
form	liquid
specific activity	377 U/mg
manufacturer/tradename	Upstate®
technique(s)	activity assay: suitable (kinase)
solubility	water: soluble
NCBI accession no.	NM_004958.2
UniProt accession no.	P42345
storage temp.	−70°C
Quality Level	100
Gene Information	human ... MTOR(2475)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.