Merck, 14-1109, Human Recombinant Histone H2A.Z

General description
Full-length human recombinant H2A.Z expressed in E. Coli and purified by Fast Protein Liquid Chromatography (FPLC ).
The histone variant H2A.Z is a evolutionarily conserved histone variant with a calculated molecular weight of13.5 kDa. This variant replaces conventional histone H2A in a subset of nucleosomes. The nucleosome is the basic unit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histones H2A, H2B, H3 and H4. The histone variant H2AZ has emerged as a key regulator of chromatin function and plays an essential role during mammalian development. H2A.Z is involved in transcriptional regulation, antisilencing, silencing, and genome stability.
Application
Suitable for in vitro chromatin assembly and enzyme activity assays
Physical form
Recombinant protein in a buffer containing 20mM Tris-Cl pH 7.6, 1mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5mM PMSF and 1mM DTT.
Preparation Note
Stable at -80ºC for 1 year from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.