Description
General description
125 kDa
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.
Immunogen
Epitope: Tyrosine 397
Generated from human FAK, a.a. 393-404, phosphorylated on Tyr397.
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18 is validated for use in WB for the detection of phospho-Focal Adhesion Kinase (Tyr397).
Biochem/physiol Actions
Reacts specifically with Focal Adhesion Kinase (FAK) when phosphorylated on Tyr397. FAK is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop.
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA, and <0.09% sodium azide.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
LPS treated RAW 264 lysates.
Evaluated by Western Blot in LPS treated RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-FAK (Tyr397) on 10 ug of LPS treated RAW 264 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-974
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
