Merck, 05-1469, Anti-NFĸB Antibody, p65, clone 1G10.2

General description
65 kDa
NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100)1, 2. NFĸB/Rel proteins exist as homo- or heterodimer complexes.
In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. Upon stimulation by multiple inducers, IĸBα is rapidly phosphorylated and degraded. This results in the release of the NFĸB complex, most commonly the p105/p65 heterodimer. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription.

Immunogen
Epitope: NLS
Synthetic peptide corresponding to the NLS region of human NFĸB p65.
Application
Anti-NFĸB Antibody, p65, clone 1G10.2 detects level of NFĸB & has been published & validated for use in WB, IF & IC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Biochem/physiol Actions
Detects NFĸB p65.
Expected to cross-react with bovine based on complete sequence homology.
Other species have not been tested.
Physical form
Format: Purified
Protein A Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note
Routinely evaluated by Western Blot.

Western Blot Analysis:
This lot detected NFB, p65 at 1:1,000 dilution in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.