Merck, 05-484, Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3

Name: Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3
Brand: Merck
SKU: 05-484

Size:100 μg, clone 20G3, Upstate®, from mouse

Available Catalog Numbers

05-0280 05-0300 05-0370 05-0390 05-0400 05-0410 05-0500 05-0535 05-0604 05-0760 05-0810 05-0860 05-0900 05-1000 05-1000X 05-1003 05-101 05-104 05-1044 05-1047 05-1049 05-1050 05-1050X

Description

General description
175kDa
Immunogen
peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
Application
Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Biochem/physiol Actions
The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
Physical form
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Preparation Note
2 years at -20°C
Analysis Note
routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
Other Notes
Replaces: 04-337; 04-341
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Specifications

General

UNSPSC Code	12352203
NACRES	NA.41
eCl@ss	32160702
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	20G3, monoclonal
species reactivity	human, mouse
manufacturer/tradename	Upstate®
technique(s)	immunoprecipitation (IP): suitable
isotype	IgG1κ
NCBI accession no.	NM_005228
UniProt accession no.	P00533
shipped in	wet ice
target post-translational modification	phosphorylation (non-phospho-Tyr1173)
Quality Level	100
Gene Information	human ... EGFR(1956) mouse ... Egfr(13649)

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