Description
General description
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
~60 kDa
Immunogen
KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to amino acids 473-488 of human HDAC2 (Accession # NP_001518) containing a N-terminal cysteine
Application
Anti-HDAC2 Antibody, clone 3F3 is a Mouse Monoclonal Antibody for detection of HDAC2 also known as YY1-associated factor 1, histone deacetylase 2 & has been validated in ICC, IP & WB.
Immunocytochemistry:
This antibody has been reported by an independent laboratory to show positive immunostaining for HDAC2 in NIH/3T3 and U2OS cells fixed with paraformaldehyde.
Immunoprecipitation and HDAC Assay:
3 μL of a previous lot was used to immunoprecipitate HDAC activity from HeLa nuclear extract, which was then measured using the HDAC Assay Kit (Fluorometric Detection) (Catalog # 17-356). An independent laboratory has also demonstrated positive immunoprecipitation /HDAC assay results using NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Biochem/physiol Actions
Predicted to cross-react with rat based on sequence homology.
Recognizes HDAC2, MW ~60 kDa.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Evaluated by western blot on nuclear extracts from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected HDAC2 in nuclear extracts from HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-229
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
