Merck, 05-902R, Anti-HA Tag Antibody, clone 114-2C-7, rabbit monoclonal

General description
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 05-902R is a recombinant version of the rabbit monoclonal Cat no. 05-902.
Immunogen
Synthetic peptide containing the sequence ...CYPYDVPDY... derived from the influenza virus hemaglutinin (HA) protein.
Application
Anti-HA Tag Antibody, clone 114-2C-7 detects level of HA Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Biochem/physiol Actions
The antibody recognizes recombinant proteins containing the HA epitope tag.
Physical form
Format: Purified
Protein A purified
Protein A purified rabbit immunoglobulin in buffer containing 60mM Tris, 90 mM NaCl, 0.03% NaN3, 60mM glycine, pH 7.5, and 40% glycerol.
Preparation Note
Maintain for 1 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Routinely evaluated by Western Blot HA-tagged WNT-1 transfected HEK293 cells.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.