Merck, 05-384, Anti-α-Actinin Antibody, clone AT6/172

General description
100 kDa
Immunogen
Human α-actinin
Application
Immunoprecipitation is not recommended
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.
Biochem/physiol Actions
Recognizes α-actinin.
Physical form
Format: Purified
Please see product datasheet for lot-specific formulation.
Protein A purified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated in immunoblot on human A431 cells
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.