Merck, 05-1424, Anti-Endoglin Antibody, clone P3D1

General description
Endoglin is a homodimeric integral membrane glycoprotein comprised of 95 kDa subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family ligands to signaling receptors. Endoglin binds TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. It is also expressed by U937 cells and activated macrophages. Endoglin expression is up regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.
~71 kDa
Immunogen
Human resting umbilical vein endothelial cells.
Application
Anti-Endoglin Antibody, clone P3D1 is an antibody against Endoglin for use in WB, IH, IC, IP, FC, ELISA.
Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue.

ELISA: A previous lot of this antibody was used in ELISA.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunocytochemistry: A previous lot of this antibody was used in IC.

Flow Cytometry: A previous lot of this antibody was used in FC.

Optimal working dilutions must be determined by the end user.

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Biochem/physiol Actions
Reacts with human endoglin.
Physical form
Format: Purified
Purified
Purified mouse monoclonal IgG2a in 0.02M phosphate buffer, 0.25M sodium chloride, 0.1% sodium azide, pH 7.6
Preparation Note
Stable at 2-8°C for up to 1 year from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ENDOGLIN on 10 μg of Jurkat lysates.

Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB2152
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.