Merck, 05-1319, Anti-EZH2 Antibody, clone BD43

General description
EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene
~98 kDa
Immunogen
Recombinant fusion protein of amino acids 1-286 of human EZH2 tagged with MBP.
Application
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Chromatin Biology
Use Anti-EZH2 Antibody, clone BD43 (Mouse Monoclonal Antibody) validated in WB to detect EZH2 also known as Lysine N-methyltransferase 6, enhancer of zeste homolog 2, ENX-1.
Biochem/physiol Actions
Not tested in other species.
This antibody recognizes human EZH2 (~98kDa).
Physical form
Format: Purified
Protein G Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note
Control
HeLa Nuclear Extracts
Western Blot Analysis: 1:5,000 from a previous lot detected EZH2 of HeLa Nuclear extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1047
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.