Description
General description
33 kDa
Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system and has a molecular mass of 21.5 kDa. MBP is believed to be important in the process of myelination of nerves in the central nervous system (CNS). MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.
The pool of MBP in the central nervous system is very diverse, with several splice variants being expressed and a large number of post-translational modifications on the protein, which include phosphorylation, methylation, deamidation and citrullination. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the Myelin sheath.
Interest in MBP has centered on its role in demyelinating diseases, particularly multiple sclerosis (MS). Several studies have shown a role for antibodies against MBP in the pathogenesis of MS. Some studies have linked a genetic predisposition to MS to the MBP gene, though a majority have not.
Immunogen
Synthetic peptide corresponding to the human myelin basic protein sequence phosphorylated at Thr98 and coupled to tuberculin
Application
Detect phospho-MBP using this Anti-phospho-MBP Antibody, clone P12 validated for use in EA & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases
Biochem/physiol Actions
Recognizes MBP phosphorylated at Threonine 98; Some preparations of MBP contain basal levels of phosphorylated MBP, which are detected by the pMBP antibody.
Physical form
Format: Purified
Protein A purified
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, PBS, and 0.09% sodium azide as a preservative.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Mouse brain extract or rat brain extract
routinely evaluated by immunoblot on MBP phosphorylated by MAP Kinase 2/Erk2, active (Catalog #14-173)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
