Merck, 05-598, Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002

General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa
Immunogen
Peptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.
Application
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.
ELISA Analysis:
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.

Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.

Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
Biochem/physiol Actions
Broad species cross-reactivity expected due to sequence conservation.
This antibody recognizes Histone H3 phosphorylated at Serine 10.
Physical form
Format: Purified
Purified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Analysis Note
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
Routinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.

Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
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