Description
General description
The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
~35 kDa
Immunogen
MBP-tagged recombinant protein corres-ponding to full length human BMI1.
Application
Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.
Chromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Biochem/physiol Actions
This antibody recognizes BMI1.
Physical form
Anti-BMI1, clone AF27
Format: Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
MEF1 cell lysate
Evaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
