Merck, 05-584, Anti-Tie2/TEK Antibody, clone Ab33

General description
TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
145 kDa
Immunogen
Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33
Application
Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.

Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
This antibody is not suitable for paraffin-embedded sections.
This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.
Biochem/physiol Actions
Recognizes Tie2/TEK, Mr 145kDa
Physical form
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
Analysis Note
Evaluated by western blot on RIPA lysates from HUVEC cells.

Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
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