Description
General description
170 kDa
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
Immunogen
Epitope: Extracelluar domain (N-term)
GST-fusion protein corresponding to amino acids 80-434 of human Met.
Application
Detect Met (extracellular) using this Anti-Met (extracellular) Antibody, clone 4F8.2 validated for use in IH(P) & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Immunohistochemistry Analysis :
Biochem/physiol Actions
Detects Met on its extracellular domain.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Analysis Note
Routinely evaluated by Western Blot.
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-237
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
