Merck, 05-545, Anti-PP2A Antibody, C subunit, clone 7A6

General description
36 kDa
Immunogen
peptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit
Application
Anti-PP2A Antibody, C subunit, clone 7A6 detects level of PP2A & has been published & validated for use in IP & WB.
Research Category
Signaling

Neuroscience
Research Sub Category
Kinases & Phosphatases

Neurodegenerative Diseases
Biochem/physiol Actions
Recognizes the catalytic subunit of PP2A.
Physical form
Cell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative.
Unpurified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431 cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.