Description
General description
67 kDa
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Immunogen
Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2
Application
Anti-Chk2 Antibody, clone 7 is a mouse monoclonal antibody for detection of Chk2 also known as CHK2 checkpoint homolog (S. pombe), serine/threonine-protein kinase CHK2 & has been validated in WB, IP, ICC.
Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Chk2 from HeLa cells.
Immunocytochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect Chk2 in HeLa cells.
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Chk2 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Chk2
Others not tested.
Predicted to cross-react with rat based on sequence homology.
Physical form
Format: Purified
Protein G Chromatography
Protein G purified mouse IgG1 buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
Preparation Note
Stable for 1 year at from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Routinely evaluated by western blot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.
Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE491
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
