Merck, 05-1403, Anti-Giα2 Antibody, clone L5

General description
Labels a single protein band of 39-42 kDa in bovine and rat membranes.
Immunogen
Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
Application
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
Biochem/physiol Actions
Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
This antibody recognizes Giα-2.
Physical form
Format: Purified
Purified
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
Huvec cell lysate
Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3077
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.