Merck, 05-414, Anti-CrkL Antibody, clone 5-6

General description
39kDa
CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.

Immunogen
Epitope: Full-length
Full length GST fusion protein corresponding to Human CRKL
Application
Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Biochem/physiol Actions
Other species have not been tested.
the C-terminal SH3 of Human CRKL
Physical form
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Analysis Note
Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells

Routinely evaluated by Western Blot on K562 cell lysate.

Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.

Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.