Merck, 05-1134, Anti-Fibroblast Growth Factor Receptor 1 Antibody, clone VBS1

General description
The fibroblast growth factor receptors are members of the tyrosine kinase superfamily and consist of an extracellular ligand domain composed of three immunoglobulin-like domains, a single transmembrane helix domain, and an intracellular domain with tyrosine kinase activity. (Ornitz, DM. and Itoh, N) Monoclonal antibodies against the high affinity tyrosine kinase Fibroblast Growth Factor (FGF) receptor may help define receptor epitopes involved in FGF binding and signal transduction which mediate coronary and tumor angiogenesis. (Venkateswaran, S., et al).
~92 kDa
Immunogen
Epitope: Extracellular
FGF Receptor purified from bovine coronary venular endothelial cells.
Application
Detect Fibroblast Growth Factor Receptor 1 using this Anti-Fibroblast Growth Factor Receptor 1 Antibody, clone VBS1 validated for use in WB, IP, IH(P), ELISA.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH(P) (Venkateswaran, S., et al)
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Biochem/physiol Actions
This antibody recognizes the extracellular ligand-binding domain of Fibroblast Growth Factor Receptor 1.
Physical form
Format: Purified
Purified mouse monoclonal IgM in 0.015 M potassium phosphate buffer containing 0.15 M NaCl, 0.1% sodium azide, pH 7.20.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.78 µg/mL of this antibody detected Fibroblast Growth Factor Receptor 1 in 10 µg of NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB125
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.