Merck, 05-1471, Anti-Rictor Antibody, clone 9F1.2

General description
190 kDa Observed
Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.
Immunogen
Recombinant protein corresponding to human Rictor.
Application
Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.
Biochem/physiol Actions
This antibody recognizes Rictor.
Physical form
Format: Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Analysis Note
Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.
Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.