Description
General description
Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
~ 266 kDa Observed
Immunogen
Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.
Application
Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human heart lysate
Evaluated by Western Blot in human heart lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-322
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
