Merck, 05-1355, Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10

General description
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.
~ 13 kDa
Immunogen
Epitope: Lys5/8/12/16
Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.
Application
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10 (mouse monoclonal antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys5/8/12/16) also known as H4KAc, Histone H4 (acetyl).
Biochem/physiol Actions
This antibody recognizes Histone H4.
Physical form
Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa acid extract lysate
Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.