Description
General description
24 kDa
Y14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
Immunogen
Epitope: Unknown
Purified human Y14.
Application
Anti-Y14 Antibody, clone 4C4 is a Mouse Monoclonal Antibody for detection of Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A & has been validated in WB, ICC & IP.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Y14 in HeLa and NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in immunoprecipitation. Kashima, I., et al. (2006). Genes & Dev. 20:355-367.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Binding Protein (RBP)
Biochem/physiol Actions
This antibody recognizes Y14.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Y14 on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
