Merck, 05-644, Anti-CAR Antibody, clone RmcB

General description
The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.
~46 kDa
Immunogen
Native human coxsackie virus-adenovirus receptor protein.
Application
Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
Biochem/physiol Actions
Recognizes CAR at MW ~46 kDa.
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.
Analysis Note
Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
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