Description
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa observed
Immunogen
Epitope: Trimethylated Lys27
Linear peptide corresponding to human Histone H3 trimethylated at Lys27.
Application
Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1 is a mouse monoclonal antibody for detection of trimethyl Histone H3 (Lys27) also known as H3K27me3, Histone H3 (tri methyl K27) & has been validated in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blot Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of modified and nonmodified peptides.
Western Blot (Snap i.d.) Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of HeLa acid extract.
Immunocytochemistry Analysis: A 1:125 dilution from a representative lot detected Histone H3 in A431 and NIH/3T3 cells.
Dot Blot Analysis: Histone peptides with various modifications (see table) were transferred to
PVDF membrane and probed with Anti-Trimethyl Histone H3 (Lys4) (1:100 dilution). Proteins were visualized using a Donkey Anti-rabbit IgG conjugated to HRP and a chemiluminescence detection systems.
Biochem/physiol Actions
This antibody recognizes Histone H3 trimethylated at Lys27.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa acid extract
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:50,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
