General description
Insulin-like growth factor 2 (UniProt: P01346; also known Insulin-like growth factor II, IGF-II, Multiplication-stimulating activity, MSA, Multiplication-stimulating polypeptide) is encoded by the Igf2 (also known Igf-2) gene (Gene ID: 24483) in rat. This gene encodes a member of the insulin family of polypeptide growth factors that is primarily involved in fetal development, influencing cellular proliferation and differentiation. It is an imprinted gene, with expression predominantly from the paternally inherited allele. It is regulated by multiple epigenetic mechanisms, including differential methylation and transcription factor binding at the IGF2-H19 locus on chromosome 11p15. It is a candidate gene for eating disorders. IGF-II is a widely expressed, secreted protein that is synthesized with a signal peptide (aa 1-24), propeptide (aa 92-180) which is subsequently cleaved off to produce the mature protein (aa 25-91). Human IGF-II shares similar structural features with IGFI, including a 62% sequence homology. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells. Dysregulation of IGF-II expression has been implicated in various diseases, including overgrowth syndromes such as Beckwith-Wiedemann syndrome and Silver-Russell syndrome, as well as in several cancers, where IGF-II promotes tumor growth and survival. (Ref.: Wilkin, F., et al. (2000). J Clin Endocrinol Metab. 85(5):2048-56; Boo, H-J., et al. (2016). Nat Commun. 7:12961; Mellott, T.J., et al. (2017). PLoS One. 12(1):e0170450; Zolov, S.N., et al. (2021). J Biol Chem. 296:100712; Birzniece, V., et al. (2022). Endocr Connect. 11(4):e210375).
~7 kDa
Immunogen
Partially purified rat IGF-II.
Application
Anti-IGF-II, clone S1F2, Cat. No. 05-166, is a mouse monoclonal antibody that detects IGF-II and is tested for use in Western Blotting and Immunohistochemistry.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Tested Applications
Western Blotting Analysis: A representative lot detected IGF-II in Western Blotting applica tion (Mellott, T.J., et al. (2017). PLoS One.12(1):e0170450; Zolov, S.N., et al. (2021). J Biol Chem.296:100712).
Immunohistochemistry Analysis: A representative lot detected IGF-II in Immunohistochemistry application (Wilkin, F., et al. (2000). J Clin Endocrinol Metab. 85(5):2048-56; Boo, H-J., et al. (2016). Nat Commun. 7:12961).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone S1F2 is a mouse monoclonal antibody that specifically detects IGF-II. This antibody has less than 10% cross-reactivity to human IGF-I.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommended storage: +2°C to +8°C.
Analysis Note
Evaluated by Western Blotting with recombinant human IGF-II.
Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected recombinant human IGF-II.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
