Merck, 05-389, Anti-Rac1 Antibody, clone 23A8

General description
21 kDa
Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
Immunogen
Recombinant protein containing the full length human Rac
Application
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

G-proteins
This Anti-Rac1 Antibody, clone 23A8 has been published in more than 40 citations and validated for use in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Rac1 protein.
Biochem/physiol Actions
Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
Physical form
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
routinely evaluated by immunoblot on rat brain microsomal protein preparation
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.